Related Experiment Video
Updated: Aug 10, 2026

Efficient Isolation Protocol for B and T Lymphocytes from Human Palatine Tonsils
Published on: November 16, 2015
Abstract:
The material lying within the crypts of 11 human palatine tonsils was studied by scanning and transmission electron microscopy. There was a mixture of desquamated epithelial cells and various non-epithelial cells, mainly lymphocytes, with some mononuclear phagocytes and plasma cells, and a few erythrocytes, neutrophil leucocytes and mast cells. Many of the cells were degenerating and there was a great deal of cellular debris. The non-epithelial cells seemed to have entered the crypts from the lining epithelium, probably by being caught up between superficial squamous epithelial cells as they sloughed into the lumen. The widespread cellular degeneration in the crypts suggested that the cells were being lost from the body and that they had no further immunological or phagocytic function. This also suggested that the first level of defence mechanisms of the tonsil was in the epithelium, not in the crypt lumen.
More Related Videos
08:25Unraveling Key Players of Humoral Immunity: Advanced and Optimized Lymphocyte Isolation Protocol from Murine Peyer's Patches
Published on: November 21, 2018
07:38Isolation of Tonsillar Mononuclear Cells to Study Ex Vivo Innate Immune Responses in a Human Mucosal Lymphoid Tissue
Published on: June 14, 2020
Related Concept Videos
Renewal of Intestinal Stem Cells
Lymphoid Cells and Tissues
Lymphoid cells consist of various types of immune system cells. These include B and T lymphocytes, which are responsible for producing antibodies and killing infected cells, respectively. Dendritic cells act as messengers between the innate and adaptive...
Primary Lymphoid Organs
The red bone marrow is a soft, spongy tissue nestled in the interior of long bones such as the humerus and femur. It is the site...
Secondary Lymphoid Organs
The spleen is a vital organ in the lymphatic system, nestled in the upper left side of the abdomen. It is composed of two primary regions: the red pulp and the white pulp, each having distinct functions. The red pulp performs a significant role in blood filtration. It efficiently purges the blood of old or damaged red blood cells and...
Cells of the Innate Immune Response
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...
Cells of the Adaptive Immune Response