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Related Experiment Videos

Fluorocarbon emulsions: methodology to assess efficacy

A L Rosen, L R Sehgal, S A Gould

    Critical Care Medicine
    |March 1, 1982
    PubMed
    Summary

    This study presents a reliable method to measure oxygen in Fluosol-DA (20%), an oxygen carrier, aiding its evaluation as a red cell substitute. The technique quantifies oxygen in perfluorochemical and aqueous phases for better clinical efficacy assessment.

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    Area of Science:

    • Biomedical Engineering
    • Cardiovascular Research
    • Blood Substitutes

    Background:

    • Fluosol-DA (20%) is an acellular oxygen carrier with potential as a red cell substitute.
    • Clinical evaluation of its efficacy necessitates understanding oxygen loading and unloading by the perfluorochemical (pfc) phase.

    Purpose of the Study:

    • To describe a method for measuring oxygen content in both the pfc and aqueous phases of Fluosol-DA.
    • To calculate the contribution of the pfc phase to total oxygen delivery and consumption (VO2).

    Main Methods:

    • Measurement of dissolved oxygen in pfc and aqueous phases using a blood gas analyzer.
    • Determination of oxygen bound to hemoglobin via co-oximetry.
    • Calculation of pfc phase oxygen contribution and fractional extraction.

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    Main Results:

    • The described technique allows for the quantification of oxygen in all relevant phases of Fluosol-DA.
    • It enables calculation of the perfluorochemical phase's role in oxygen delivery and consumption.
    • The fractional oxygen extraction in the pfc phase can be determined.

    Conclusions:

    • A simple and reliable method is established for evaluating oxygen transport by Fluosol-DA.
    • This technique facilitates the clinical assessment of fluorocarbon emulsions as red cell substitutes.
    • Accurate measurement of oxygen dynamics is crucial for understanding the efficacy of artificial oxygen carriers.