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Cryopreservation of the human multipotent stem cell

I Fabian, D Douer, J R Wells

    Experimental Hematology
    |January 1, 1982
    PubMed
    Summary

    Cryopreservation of human bone marrow cells effectively preserves their ability to form myeloid, erythroid, and mixed hematopoietic colonies. Post-thaw recovery rates for myeloid (CFUGM) and erythroid (BFUE) progenitors were high, demonstrating cryopreservation

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    Area of Science:

    • Hematology
    • Cell Biology
    • Cryobiology

    Background:

    • Human bone marrow contains hematopoietic stem and progenitor cells essential for blood formation.
    • Cryopreservation is a critical technique for preserving biological materials, including stem cells.

    Purpose of the Study:

    • To evaluate the in vitro colony-forming capacity of human bone marrow cells after cryopreservation.
    • To assess the recovery and function of myeloid (CFUGM) and erythroid (BFUE) progenitors post-thaw.

    Main Methods:

    • Light density human bone marrow cells were cryopreserved and subsequently thawed.
    • Cells were cultured in vitro to assess colony formation (CFUGM, BFUE, mixed colonies).
    • Proliferation of mixed colonies was supported by T-lymphocyte conditioned media and erythropoietin.

    Main Results:

    • Cryopreserved bone marrow cells retained their capacity to form myeloid, erythroid, and mixed hematopoietic colonies.
    • All mixed colonies contained neutrophil and erythroid precursors, with 25% including mononuclear phagocytes.
    • Recovery rates for CFUGM and BFUE were 76% and 72%, respectively, indicating minimal loss.

    Conclusions:

    • Cryopreservation is an effective method for preserving the hematopoietic progenitor potential of human bone marrow.
    • The study demonstrates the feasibility of using cryopreserved bone marrow for future hematopoietic cell culture and research.
    • High recovery rates suggest cryopreservation does not significantly impair the differentiation capacity of key hematopoietic progenitors.

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