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Related Experiment Videos

Radiation effect on partially synchronized Yoshida sarcoma cells

P H Bippus, J Heitz, U Rühl

    Journal of Cancer Research and Clinical Oncology
    |January 1, 1982
    PubMed
    Summary

    Radiation therapy affects Yoshida sarcoma cell growth, with dose-dependent inhibition. While cell cycle phase at irradiation showed minimal impact on DNA synthesis and mitotic index, G2-phase irradiation significantly reduced in vivo growth short-term.

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    Area of Science:

    • Cell Biology
    • Radiation Oncology
    • Cancer Research

    Background:

    • Yoshida sarcoma cells exhibit growth characteristics similar to ascites cells.
    • Understanding radiation effects on cancer cells is crucial for effective therapy.

    Purpose of the Study:

    • To investigate the impact of radiation on Yoshida sarcoma cell growth and DNA synthesis.
    • To determine if cell cycle synchronization influences radiation sensitivity.
    • To evaluate the effect of irradiation timing within the cell cycle on tumor growth.

    Main Methods:

    • Partial synchronization of Yoshida sarcoma cells in vitro using excess thymidine.
    • Irradiation of synchronized and non-synchronized cells at various doses.
    • Measurement of 3H-thymidine incorporation into DNA.

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  • Analysis of mitotic index and cell growth in vitro and in vivo.

    • Main Results:

    • Radiation inhibited cell growth in a dose-dependent manner, with 50% inhibition at 380 rad (in vivo) and 480 rad (in vitro).
    • DNA synthesis (3H-thymidine incorporation) was reduced by 10-32% post-irradiation.
    • While most cell cycle phases showed similar responses, irradiation in the G2-phase led to significantly reduced in vivo growth at 48 hours.
    • Differences diminished with longer culture periods (>72 hours).

    Conclusions:

    • Radiation sensitivity of Yoshida sarcoma cells is dose-dependent.
    • Irradiation in the G2-phase has a more pronounced short-term negative impact on in vivo tumor growth compared to other phases.
    • Cell cycle phase-specific irradiation effects are more evident in short-term in vivo growth than in vitro parameters like DNA synthesis or mitotic index.