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Updated: Aug 19, 2026

Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells
06:29

Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells

Published on: January 29, 2014

Hemolytic efficiency of cell-bound IgM: evidence that IgM-C1 complexes activate C4 molecules not hemolytic with

R Ejzemberg, T Borsos

    Journal of Immunology (Baltimore, Md. : 1950)
    |April 1, 1982
    PubMed

    The hemolytic efficiency of rabbit-anti-Forssman IgM antibody depends on the source of complement components. With whole guinea pig complement as few as one in 10 IgM molecules was hemolytic; it was found that with purified guinea pig C1, C4, and C2 components and with rat late-acting components, all IgM molecules were hemolytic. Evidence indicates that the block occurred as a result of generating at least two different cell-bound C4 molecules, one of which was not competent with homologous late-acting components.

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