Quantitation of lipophilic chloroethylnitrosourea cancer chemotherapeutic agents

A simple and rapid quantitative method for the derivatization and determination of lipophilic chloroethylnitrosoureas is described. This procedure involves the ether extraction of the chloroethylnitrosourea from plasma and conversion of the parent drug to an O-methylcarbamate by reaction in anhydrous methanol. The product O-methylcarbamate may be separated with gas chromatography (GC) and detected with nitrogen-specific GC detectors or with mass spectrometry using multiple-ion detection. The lower limit of detection for each method was approximately 100 ng/ml plasma.