Related Experiment Video
Updated: Aug 13, 2026

The Cell-based L-Glutathione Protection Assays to Study Endocytosis and Recycling of Plasma Membrane Proteins
Published on: December 13, 2013
Glucosylated albumin and its influence on salicylate binding
Abstract:
Human serum albumin was incubated at 37 degrees in 0.01 M phosphate buffer (pH 7.4) under sterile conditions for up to 10 days with labeled [14C]glucose (1-25 mg/ml). Glucose incorporated into albumin was calculated following extensive dialysis of the incubation mixture. The results indicated that glucose reacted with albumin by a nonenzymatic process involving Schiff base formation and Amadori rearrangement to a stable ketoamine derivative. The degree of glucosylation was dependent on the reaction time, glucose concentration, and pH. Glucosylation was enhanced when albumin was fatty acid free. Glucosylated albumin was separated from unmodified albumin by cation exchange chromatography on carboxymethylcellulose and quantitated colorimetrically with 2-thiobarbituric acid. Salicylate binding studies revealed that the glucosylated component had a decreased salicylate binding capacity accompanied by a reduction in the number of classes of binding sites.
Related Concept Videos
Drug Distribution: Plasma Protein Binding
Drug Metabolism: Phase II Reactions
Drug Binding to Blood Components
HSA is the most abundant plasma protein and is vital in drug binding. It contains distinct drug-binding sites, with different drugs exhibiting affinity for specific sites. There are three main drug-binding domains for HSA: sites I, II, and III. These domains are further...
Factors Affecting Protein-Drug Binding: Drug-Related Factors
One crucial factor in drug-protein binding is the drug's lipophilicity or its affinity for fat. More lipophilic drugs tend to have higher binding extents. For example, highly lipophilic drugs like cloxacillin exhibit substantial protein binding, with as much as 95% of the drug binding to proteins. In contrast,...
Factors Affecting Protein-Drug Binding: Protein-Related Factors
The physicochemical properties of a drug play a significant role in its ability to bind to proteins. Lipophilic drugs, which dissolve in fats, oils, and lipids, can be bound by...
Factors Affecting Protein-Drug Binding: Patient-Related Factors
Age stands as a key determinant in protein-drug binding. Neonates, characterized by low albumin content, experience heightened concentrations of unbound drugs such as phenytoin and...

