[Improved method of perfusion and functional loading of the isolated heart. Studies as a control and following...

The authors have elaborated and studied in 52 experiments an improved method of donor perfusion of an isolated dog heart. The technique of removing the heart without ischaemia and after cardioplegia as well as the technique of perfusion of the arrested and the contracting heart are described. The authors used their own method of the functional load testing of the left ventricle after cardioplegia. The function of the isolated dog heart without ischaemia was studied in control experiments. The advantages of the described method of perfusion and functional load of an isolated heart are: it is manageable, physiological and easily reproduced. The method can be used in experimental cardiology.