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Spiroplasmas: cultivation in chemically defined medium

C J Chang, T A Chen

    Science (New York, N.Y.)
    |February 26, 1982
    PubMed
    Summary
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    A new chemically defined medium, CC-494, successfully supports in vitro cultivation of multiple spiroplasma species, including flower and honey bee variants. While growth rates are slower than in undefined media, maximal populations are comparable, indicating its potential for spiroplasma research.

    Area of Science:

    • Microbiology
    • Infectious Diseases
    • Bacteriology

    Background:

    • Spiroplasmas are helical, wall-less bacteria that can cause diseases in plants and insects.
    • Cultivating spiroplasmas in vitro is crucial for studying their biology and developing control strategies.
    • Undefined media, like C-3G, have been traditionally used but lack precise control over nutritional components.

    Purpose of the Study:

    • To evaluate the efficacy of a novel chemically defined medium (CC-494) for the in vitro cultivation of spiroplasmas.
    • To compare the growth performance of specific spiroplasma strains in the defined medium versus an undefined medium.

    Main Methods:

    • In vitro cultivation of spiroplasma strains (flower spiroplasmas FS 23-6, FS SR-3; honey bee spiroplasma HBS AS-576) was performed.

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  • Growth was assessed in a chemically defined medium (CC-494) and compared to an undefined medium (C-3G).
  • Maximal populations and growth rates were determined for each spiroplasma in both media.

    • Main Results:

    • The chemically defined medium CC-494 supported the in vitro cultivation of three distinct spiroplasma serogroups.
    • Maximal populations achieved in CC-494 were comparable to those in the undefined medium C-3G for the tested spiroplasmas.
    • Slower growth rates were observed for all three spiroplasma strains when cultured in the defined medium CC-494.

    Conclusions:

    • Chemically defined medium CC-494 is a viable option for the in vitro cultivation of specific spiroplasma species.
    • While growth rates may be reduced, the defined nature of CC-494 offers advantages for controlled spiroplasma research.
    • Further optimization of CC-494 may enhance growth rates, making it a more robust tool for spiroplasma studies.