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Evaluation of a thrombin-sensitive fluorogenic substrate for heparin concentration in plasma
Heparin levels in 76 heparinized patient plasma samples were determined with a thrombin-sensitive fluorogenic substrate. Results were compared with heparin values derived from an activated partial thromboplastin time test. The correlation of heparin concentrations obtained by these two methods was poor (r = 0.56). The amidolytic assay gave higher heparin values than the clotting method. The discrepancy is attributed to factors inherent to the methodology of these assay systems.
Heparin levels in 76 heparinized patient plasma samples were determined with a thrombin-sensitive fluorogenic substrate. Results were compared with heparin values derived from an activated partial thromboplastin time test. The correlation of heparin concentrations obtained by these two methods was poor (r = 0.56). The amidolytic assay gave higher heparin values than the clotting method. The discrepancy is attributed to factors inherent to the methodology of these assay systems.