Estimation of the saturation of serum transferrin by an electrophoretic technique

An electrophoretic method is described for estimating the saturation of serum transferrin. After first precipitating most of the other proteins with Rivanol, the apo-, mono-, and di-ferric transferrins are separated by electrophoresis in urea-polyacrylamide gel. Densitometric scanning enables the percentage saturation to be calculated.