[Isolation and study of the compounds of a peptide nature contained in an oleandomycin preparation]

Marketed oleandomycin phosphate, as well as the International Standard of oleandomycin (Great Britain, the 1st Int. Std., 1964) were studied with a purpose of determining their composition. The components were separated by liquid chromatography on columns with Sefadex LH-20 using 50 per cent ethanol as an eluent. The Lowry method using the biuret test and assays of the amino acids in the hydrolysates were used to show for the first time that marketed oleandomycin and its International Standard contained an admixture of the peptide nature. The data of the amino acid assays suggest that the admixture and oleandomycin is a fixed complex (adduct).