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Collagen synthesis by bone marrow stromal cells: a quantitative study

S A Bentley

    British Journal of Haematology
    |March 1, 1982
    PubMed
    Summary
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    Marrow-derived adherent cells (MDAC) in culture significantly increase collagen synthesis, supporting hematopoietic stem cell (HSC) proliferation. These findings highlight specific collagen-producing bone marrow connective tissue cells.

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Hematology

    Background:

    • Marrow-derived adherent cells (MDAC) create a microenvironment supporting hematopoietic stem cell (HSC) proliferation in vitro.
    • Understanding the collagen synthesis of these cells is crucial for comprehending bone marrow's supportive functions.

    Purpose of the Study:

    • To quantitatively investigate the relationship between collagen synthesis in intact bone marrow and in MDAC cultures.
    • To characterize the collagen types and ratios produced by MDAC.

    Main Methods:

    • Quantitative analysis of collagen synthesis as a fraction of total protein synthesis in fresh murine bone marrow cells and cultured MDAC.
    • Assessment of collagen types I and III synthesis ratio.
    • Evaluation of MDAC's ability to support HSC proliferation in continuous bone marrow culture.

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    Main Results:

    • Collagen synthesis in fresh bone marrow cells was 0.22% of total protein synthesis, with collagen types I and III in a ~5:1 ratio.
    • MDAC cultures showed a significant increase in collagen synthesis fraction to 2.3%, maintaining the type I to III ratio.
    • MDAC monolayers supported HSC proliferation for at least 5 weeks in culture.

    Conclusions:

    • MDAC cultures select for specific bone marrow connective tissue cells, likely reticulum cells.
    • These selected cells are major producers of collagen types I and III in a consistent ratio.
    • MDAC cultures provide a valuable model for studying the supportive microenvironment of HSCs.