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The effect of growth media upon the ultrastructure of Blastomyces dermatitidis

M H Zahraee, T E Wilson, E R Scheer

    Canadian Journal of Microbiology
    |February 1, 1982
    PubMed

    Insights

    Culture media significantly impact Blastomyces dermatitidis ultrastructure. Leucine-glucose (LG) medium promoted granular cytoplasm and defined membranes in yeast and mycelial forms, unlike brain heart infusion (BHI) medium.

    Area of Science:

    • Mycology
    • Cell Biology
    • Medical Mycology

    Background:

    • Blastomyces dermatitidis is a fungus causing blastomycosis.
    • Fungal morphology is influenced by culture conditions.
    • Understanding ultrastructural changes is key to fungal biology.

    Purpose of the Study:

    • To compare the ultrastructure of Blastomyces dermatitidis in different culture media.
    • To investigate how nutrient composition affects fungal cell morphology.
    • To identify media-specific ultrastructural characteristics.

    Main Methods:

    • Culturing Blastomyces dermatitidis in Brain Heart Infusion (BHI), Leucine-Glucose (LG), Yeast Extract-Peptone (YP), and Glucose-Yeast Extract-Peptone (GYP) media.
    • Comparative ultrastructural analysis using electron microscopy.
    • Observing yeast, intermediate, and mycelial forms.

    Main Results:

    • Leucine-glucose (LG) medium resulted in more granular cytoplasm and defined membranes compared to BHI.
    • LG-grown yeasts had increased glycogen and fewer lipid bodies; some exhibited bizarre shapes.
    • YP-grown yeasts showed more defined internal structures, including double budding and elongation.
    • GYP-grown yeasts lacked defined structures, had less glycogen, and contained large vacuoles.
    • Intrayeast hyphae were exclusively observed in BHI-grown intermediate cells.

    Conclusions:

    • Culture media composition profoundly alters Blastomyces dermatitidis ultrastructure.
    • LG medium supports distinct morphological features in yeast and mycelial forms.
    • YP and GYP media induce unique yeast morphologies, differing from BHI.
    • These findings highlight the plasticity of B. dermatitidis morphology in response to environmental cues.

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