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Oxygen-transporting fluids and oxygen delivery with hemodilution
Critical Care Medicine
|April 1, 1982
Summary
Stroma-free hemoglobin (SFH) solutions reversed hemodynamic issues after severe blood loss in dogs. However, short intravascular persistence limited cardiac output increase, though pyridoxalation improved oxygen unloading.
Area of Science:
- Biomedical Engineering
- Hematology
- Physiology
Background:
- Severe blood loss causes significant hemodynamic disorders.
- Stroma-free hemoglobin (SFH) solutions are potential blood substitutes.
- Assessing hemodynamic effectiveness and oxygen characteristics of SFH is crucial.
Purpose of the Study:
- To evaluate the hemodynamic effectiveness of stroma-free hemoglobin (SFH) solutions.
- To investigate the oxygen characteristics of SFH and pyridoxalated SFH-PLP.
- To determine the intravascular persistence of SFH-PLP.
Main Methods:
- Eleven dogs underwent severe blood withdrawal (two-thirds of blood volume) and replacement with SFH or polyhemoglobin (SFH-PLP).
- Ringer's lactate was administered to maintain right atrial pressure.
- Five dogs were used to study the intravascular persistence of 131I-labeled SFH-PLP.
Main Results:
- Hemodynamic disorders were reversed by hemoglobin solution infusion.
- Short intravascular persistence of SFH-PLP limited cardiac output increase.
- Pyridoxalation of hemoglobin reduced oxygen affinity, enhancing tissue oxygen unloading.
Conclusions:
- Hemoglobin solutions can reverse hemodynamic instability post-hemorrhage.
- The short intravascular half-time of SFH-PLP necessitates strategies like crosslinking to improve persistence.
- Further research is needed to establish polyhemoglobin as a long-term blood substitute.