Quantitative determination of fluorescein in the anterior chamber and vitreous body

The blood-retinal barrier plays an important role in the physiology of the retina, the vitreous body and the aqueous humour. Many intra-ocular processes cause a breakdown of this barrier and this results in an increase in the concentration of fluorescein in the vitreous and the aqueous after it has been injected intravenously (Cunha-Vaz et al., 1975). In our clinic we have built an apparatus for the quantitative determination of the concentration of this fluorescein in both healthy and pathological eyes (Brubaker and Coakes, 1978). The goal of the present communication is to invite other workers with similar interests to compare their results with ours.