Release of enzymes from cultured fibroblasts induced by lysophosphatidylcholine

Release of enzymes from cultured fibroblasts was induced by exposure to the action of lysophosphatidylcholine (LPC). The effect of LPC was dependent on cell density. At identical densities a linear relationship between the concentration of LPC and the efflux of enzymes was found. Efflux of lactate dehydrogenase occurred at LPC concentrations of 5-10 micrograms/ml. Leakage of aspartate aminotransferase took place at higher concentrations, 10-25 micrograms/ml. Efflux of glutamate dehydrogenase was observed only at high concentrations of LPC, approximately 50 micrograms/ml. The efflux of the two former enzymes decreased with time.