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Biochemical changes induced in human plasma by its lipoprotein lipase
The International Journal of Biochemistry
|January 1, 1982
Summary
Lipoprotein lipase in human plasma significantly decreases calcium, triglycerides, and phosphorus levels. These enzymatic hydrolysis effects mimic changes seen during plasma storage.
Area of Science:
- Biochemistry
- Clinical Chemistry
- Enzymology
Background:
- Lipoprotein lipase (LPL) is a key enzyme in lipid metabolism.
- Plasma composition changes during storage, impacting its biochemical parameters.
- Understanding LPL activity is crucial for interpreting plasma analyses.
Purpose of the Study:
- To investigate the biochemical changes in human plasma induced by purified lipoprotein lipase.
- To compare these in vitro changes to those observed during plasma storage.
Main Methods:
- Human citrated plasma was incubated with purified lipoprotein lipase concentrate.
- Incubation was performed for 6 hours at 37°C and pH 7.0.
- Concentrations of various plasma components (calcium, triglycerides, phosphorus, fatty acids) were measured before and after incubation.
Main Results:
- Incubation with lipoprotein lipase markedly decreased calcium, triglycerides, total phosphorus, lipid phosphorus, and total fatty acids.
- A concurrent increase in non-esterified fatty acids and inorganic phosphorus was observed, indicating enzymatic hydrolysis.
- The observed changes were quantitatively similar to those reported in plasma stored for 1-2 months at room temperature.
Conclusions:
- Lipoprotein lipase actively hydrolyzes triglycerides and lipid phosphorus in human plasma.
- These enzymatic actions lead to significant alterations in plasma biochemistry.
- The in vitro effects of lipoprotein lipase closely resemble biochemical changes occurring during prolonged plasma storage.
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