Age-related changes in rat glomerular basement membrane

1. The yield of GBM from rat kidney increased with rat age and the membrane became more collagen-like, as judged by the glycine, hydroxyproline, hydroxylysine content and the degree of glycosylation. 2. GBM became progressively less soluble in sodium dodecyl sulphate (SDS) and 2-mercaptoethanol with age. An increase in the lower molecular weight subunits of membranes prepared from older rats was demonstrated by polyacrylamide gel electrophoresis.