Purification of muscle acyl-CoA dehydrogenases extracted from Astacus fluviatilis pallipes

1. Three acyl-CoA dehydrogenases were purified from muscles of Astacus fluviatilis. 2. The electrophoretic pattern of these enzymes was close to the one reported in the case of the same proteins coming from mammals. 3. Each of the acyl-CoA dehydrogenases extracted showed a great specificity of substrate. 4. These enzymes had no activity when tested in the absence of a protein purified from the crayfish muscles, which has the same electrophoretic pattern as the electron transferring protein of mammals.