Direct fluorometric determination of cyanide in human materials

A detailed procedure of a new simple fluorometric assay for cyanide in human blood, stomach contents and urine is presented. This method requires neither diffusion nor distillation of cyanide from samples; small amounts of the samples were directly added to incubation mixtures, which were then heated at 80 degrees C for 45 min and acidified for fluorescence measurements. Our method is much more rapid than the previous ones. Some data are presented on specificity and sensitivity of the present method.