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Related Experiment Videos

Human complement (C3b) receptors defined by a mouse monoclonal antibody

J Gerdes, M Naiem, D Y Mason

    Immunology
    |April 1, 1982
    PubMed
    Summary

    A new monoclonal antibody, C3RTo5, selectively targets human complement C3b receptors. This antibody inhibits C3b binding and identifies a 205,000 molecular weight glycoprotein, confirming its specificity for C3b receptors.

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    Area of Science:

    • Immunology
    • Molecular Biology
    • Cell Biology

    Background:

    • Complement system plays a crucial role in immune response.
    • Complement receptors (CRs) mediate various immune functions.
    • Specific antibodies are needed to study CRs, particularly C3 receptors.

    Purpose of the Study:

    • To develop and characterize a monoclonal antibody targeting human C3 receptors.
    • To investigate the specificity and binding characteristics of the new antibody.
    • To identify the molecular weight of the C3b receptor.

    Main Methods:

    • Monoclonal antibody production and characterization.
    • Ligand binding inhibition assays.
    • Immunoprecipitation and Western blotting.

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  • Flow cytometry and cell absorption studies.

    • Main Results:

    • The monoclonal antibody C3RTo5 specifically binds to human C3b receptors, inhibiting ligand binding.
    • C3RTo5 does not affect C3bi or C3d receptor binding.
    • A 205,000 molecular weight glycoprotein was identified as the C3b receptor using C3RTo5.

    Conclusions:

    • C3RTo5 is a highly selective monoclonal antibody for human C3b receptors.
    • The study confirms the presence of a 205,000 molecular weight C3b receptor on human cells.
    • C3RTo5 is a valuable tool for studying the complement system and C3b receptor function.