Cytopathogenicity of Naegleria gruberi for rat neuroblastoma cell cultures

Amoebae of Naegleria gruberi were cytopathic for cultures of rat neuroblastoma (B-103) cells. N. gruberi grew and destroyed B-103 cells at 30 degrees C. As few as one amoeba inoculated per million B-103 cells resulted in cytopathogenicity after extensive growth of N. gruberi.