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Related Experiment Videos

Automatic lactate determination by flow injection analysis

U Rydevik, L Nord, F Ingman

    International Journal of Sports Medicine
    |February 1, 1982
    PubMed
    Summary

    Flow injection analysis accurately quantifies lactate in serum using the NADH-LDH method. This validated technique offers reliable results for lactate concentrations between 0.2-20 mmoles per litre.

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    Area of Science:

    • Clinical Chemistry
    • Analytical Chemistry
    • Biochemistry

    Background:

    • Lactate is a key biomarker in various physiological and pathological conditions.
    • Accurate lactate measurement in serum is crucial for clinical diagnosis and monitoring.
    • Existing methods may have limitations in speed, precision, or sample handling.

    Purpose of the Study:

    • To develop and validate a flow injection analysis (FIA) method for serum lactate determination.
    • To assess the suitability of the NADH-LDH enzymatic method within an FIA system.
    • To compare the performance of the FIA method against a standard manual procedure.

    Main Methods:

    • Utilized flow injection analysis (FIA) for automated sample processing.
    • Employed the enzymatic nicotinamide adenine dinucleotide (NADH) and lactate dehydrogenase (LDH) reaction for lactate quantification.
    • Tested the method with precipitated serum samples to evaluate its robustness.

    Main Results:

    • The FIA method demonstrated suitability for lactate determination in the concentration range of 0.2-20 mmoles per litre.
    • Results obtained from the FIA method showed good agreement with a manual routine procedure.
    • The NADH-LDH method, when integrated with FIA, provided reliable and consistent lactate measurements.

    Conclusions:

    • Flow injection analysis provides an efficient and accurate platform for serum lactate measurement.
    • The NADH-LDH method is well-suited for FIA-based lactate determination in clinical settings.
    • This validated FIA procedure offers a reliable alternative to manual methods for routine lactate analysis.

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