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Bone marrow processing and cryopreservation

D C Linch, L J Knott, K G Patterson

    Journal of Clinical Pathology
    |February 1, 1982
    PubMed
    Summary
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    The Hemonetics 30 cell separator, using a paediatric pheresis set, best concentrated bone marrow progenitor cells before cryopreservation. This method achieved high nucleated and progenitor cell recovery after freeze-thawing.

    Area of Science:

    • Hematology
    • Cell Biology
    • Cryobiology

    Background:

    • Cryopreservation of bone marrow progenitor cells is crucial for transplantation.
    • Efficient cell concentration methods are needed to optimize cryopreservation outcomes.
    • Comparing different concentration techniques is essential for clinical applications.

    Purpose of the Study:

    • To compare three closed-system methods for concentrating bone marrow progenitor cells.
    • To determine the most effective method for cell recovery prior to cryopreservation.
    • To evaluate cell recovery rates after freeze-thawing using the optimal concentration technique.

    Main Methods:

    • Manual double centrifugation method.
    • Hemonetics 30 cell separator (paediatric pheresis set).

    Related Experiment Videos

  • Aminco Celltrifuge.

    • Main Results:

    • The Hemonetics 30 cell separator with a paediatric pheresis set yielded the best results.
    • Bone marrow was cryopreserved in 120 ml aliquots using a programmed freezer.
    • Average nucleated cell recovery was approximately 50%, and progenitor cell recovery was 80% after freeze-thawing.

    Conclusions:

    • The Hemonetics 30 cell separator is the preferred method for concentrating bone marrow progenitor cells.
    • Optimal cryopreservation protocols involve programmed freezing with rapid cooling during phase change.
    • High progenitor cell recovery rates are achievable with this optimized concentration and freezing method.