Jove
Visualize
Contact Us

Related Experiment Videos

Carboxyhaemoglobin: a possible reference material for haemoglobin assay

J M Rideout, A Louderback

    Journal of Clinical Pathology
    |March 1, 1982
    PubMed
    Summary

    A stable haemolysate is needed for remote haemoglobin testing quality control. Carboxyhaemoglobin in a haemolysate, stored under nitrogen, shows promise as a reliable reference material for haemoglobin assays.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    A sensitive and specific high performance liquid chromatographic assay for imidazole dipeptides and 3-methylhistidine in human muscle biopsies, serum and urine.

    Biomedical chromatography : BMC·1992
    Same author

    Identification of peroxyacetic acid uroporphyrin I in the urine of patients with congenital erythropoietic porphyria by liquid chromatography and mass spectrometry.

    Biomedical chromatography : BMC·1991
    Same author

    Microsomal delta 9, delta 6 and delta 5 desaturase activities and liver membrane fatty acid profiles in alcohol-fed rats.

    Biomedical chromatography : BMC·1990
    Same author

    When duplicate testing leads to different results.

    MLO: medical laboratory observer·1990
    Same author

    Isolation and characterization of beta-hydroxypropionic acid- and hydroxyacetic acid-uroporphyrin I in the urine of a patient with congenital erythropoietic porphyria by high performance liquid chromatography and liquid secondary ion mass spectrometry.

    Biomedical chromatography : BMC·1990
    Same author

    Possible role of acetaldehyde in ethanol-related rectal cocarcinogenesis in the rat.

    Gastroenterology·1990
    JoVE
    x logofacebook logolinkedin logoyoutube logo
    ABOUT JoVE
    OverviewLeadershipBlogJoVE Help Center
    AUTHORS
    Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
    LIBRARIANS
    TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
    RESEARCH
    JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
    EDUCATION
    JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
    Terms & Conditions of Use
    Privacy Policy
    Policies

    Area of Science:

    • Clinical Chemistry
    • Laboratory Medicine
    • Hematology

    Background:

    • Accurate haemoglobin estimation is crucial for diagnosing and monitoring various medical conditions.
    • Current commercially available haemolysates lack the necessary stability for reliable quality control in remote laboratory settings.
    • Inter- and intralaboratory quality control procedures are essential for ensuring the accuracy of diagnostic tests.

    Purpose of the Study:

    • To evaluate the stability of oxyhaemoglobin and carboxyhaemoglobin in an ethanediol-containing haemolysate over a three-year period.
    • To identify a suitable, rugged control material for haemoglobin estimation in remote laboratories.
    • To propose a candidate reference material for haemoglobin assay standardization.

    Main Methods:

    • Preparation of an ethanediol-containing haemolysate.
    • Longitudinal stability testing of oxyhaemoglobin and carboxyhaemoglobin forms of the haemolysate over three years.
    • Assessment of haemolysate stability under controlled conditions (e.g., under nitrogen).

    Main Results:

    • The study monitored the stability of specific haemoglobin forms over an extended period.
    • Data on the degradation or preservation of haemoglobin analytes were collected.
    • The stability characteristics of the prepared haemolysate were determined.

    Conclusions:

    • Ethanediol-containing haemolysate exhibits varying stability depending on the haemoglobin form and storage conditions.
    • Carboxyhaemoglobin, when stored under a nitrogen atmosphere, demonstrated significant stability.
    • Carboxyhaemoglobin in a stabilized haemolysate is proposed as a potential reference material for haemoglobin assays, addressing the need for rugged controls in remote labs.

    Related Experiment Videos