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Related Experiment Videos

Cutaneous sialidase

P D Mier, H van Rennes, P E van Erp

    The Journal of Investigative Dermatology
    |April 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    A new fluorometric assay quantifies skin sialidase activity. While typically similar to normal skin, some psoriasis biopsies showed significantly elevated enzyme levels.

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    Area of Science:

    • Biochemistry
    • Enzymology
    • Dermatology

    Background:

    • Sialidases are enzymes that cleave sialic acids.
    • Cutaneous sialidases play roles in skin physiology and pathology.
    • Characterization of skin sialidase is important for understanding its function.

    Purpose of the Study:

    • To develop and validate a fluorometric microassay for quantifying sialidase activity in skin.
    • To characterize the kinetic properties of cutaneous sialidase.
    • To investigate sialidase levels in normal and psoriatic skin biopsies.

    Main Methods:

    • Development of a fluorometric microassay using sialyllactose as the natural substrate.
    • Enzyme activity measurement in skin homogenates.
    • Kinetic analysis including pH optimum and Michaelis constant (Km) determination.

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  • Comparison of enzyme levels in normal and psoriatic skin samples.

    • Main Results:

    • The fluorometric microassay effectively characterized and quantified cutaneous sialidase.
    • Sialidase was localized to the particulate fraction of skin homogenates.
    • The enzyme exhibited a pH optimum of 4.0 and a Km of 0.47 mM, consistent with mammalian sialidases.
    • Psoriatic biopsies showed similar modal sialidase activity to normal skin, but a subset displayed exceptionally high levels.

    Conclusions:

    • A sensitive fluorometric assay for cutaneous sialidase is established.
    • Cutaneous sialidase shares properties with sialidases from other mammalian tissues.
    • Elevated sialidase activity in a subset of psoriatic skin warrants further investigation into its role in the disease.