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[Cytochrome composition of Acinetobacter calcoaceticus]
Acta Biologica Et Medica Germanica
|January 1, 1978
Summary
Acinetobacter calcoaceticus utilizes various carbon sources, with cytochrome b and o content remaining stable. Yeast extract boosts cytochrome d, but cytochromes are not key to n-alkane hydroxylation.
Area of Science:
- Microbiology
- Biochemistry
- Cell Biology
Context:
- Investigating the role of cytochromes in bacterial metabolism.
- Understanding the respiratory chain components in Acinetobacter calcoaceticus.
- Examining the impact of different carbon sources on bacterial cell composition.
Purpose:
- To determine the qualitative and quantitative composition of cytochromes in Acinetobacter calcoaceticus.
- To analyze how different carbon sources affect cytochrome content.
- To ascertain the involvement of cytochromes in n-alkane hydroxylation.
Summary:
- Spectrophotometric methods revealed Acinetobacter calcoaceticus contains cytochrome b, cytochrome o, and minor cytochrome d.
- Cytochrome b and o levels were consistent across various carbon sources (hexadecane, acetate, succinate, malate, yeast extract).
- Yeast extract uniquely increased cytochrome d while decreasing cytochrome o, suggesting metabolic flexibility.
Impact:
- Provides insights into the respiratory chain of Acinetobacter calcoaceticus.
- Suggests cytochromes are not directly involved in the n-alkane hydroxylation pathway in this bacterium.
- Highlights the adaptability of bacterial metabolism in response to nutrient availability.