[Reassociation kinetics of the DNA from rat liver nuclei of varying ploidy]

Using sucrose gradient centrifugation, isolated liver cell nuclei were fractionated according to different ploidy classes. DNA was isolated from the di- and polyploid as well as from the total nuclear fractions, and the kinetics of their reassociation was investigated. A similar mode of reassociation was revealed for DNAs isolated from the nuclei of different ploidy classes, testifying in favour of the true polyploidy of liver cell nuclei.