Ultrathinfilm immunoelectrophoresis

Immunoelectrophoresis (Laurell-rocket) in ultrathinfilm gels has been applied to the determination of human serum albumin in a model study. Agarose gels of 0.24 mm thickness were used and optimum electrophoretic conditions were determined. Precision was assessed and comparison with conventional immunoelectrophoresis and a chemical method was made. Though precision was poor (CV 4-6%) improvements in dispensing small volumes (0.2 microliter) should change this. Good correlation between methods (correlation coefficient 0.89-0.97) was obtained. The advantages of the technique are speed of analysis and low reagent costs.