Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Rapid radiometric serum test for antibiotic activity

R G D'Antonio, E E Camargo, T Gedra

    Antimicrobial Agents and Chemotherapy
    |February 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    A Window on Latin America.

    European journal of nuclear medicine·2016
    Same author

    Gastric emptying of water in children with severe functional fecal retention.

    Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas·2013
    Same author

    Radioguided Intraoperative Margins Evaluation (RIME): preliminary results of a new technique to aid breast cancer resection.

    European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·2007
    Same author

    Voxel-based morphometry of brain SPECT can detect the presence of active central nervous system involvement in systemic lupus erythematosus.

    Rheumatology (Oxford, England)·2006
    Same author

    Major salivary gland damage in allogeneic hematopoietic progenitor cell transplantation assessed by scintigraphic methods.

    Bone marrow transplantation·2006
    Same author

    99mTc-sestamibi thyroid uptake in euthyroid individuals and in patients with autoimmune thyroid disease.

    European journal of nuclear medicine and molecular imaging·2005

    A new 4-hour radiometric method accurately predicts antibiotic effectiveness by measuring microbial metabolic activity. This rapid technique shows high concordance with traditional methods, offering a faster way to determine bacteriostatic endpoints for clinical samples.

    Area of Science:

    • Microbiology
    • Radiochemistry
    • Clinical Diagnostics

    Background:

    • Accurate determination of antibiotic susceptibility is crucial for effective patient treatment.
    • Traditional methods like the tube dilution method can be time-consuming.
    • There is a need for faster, reliable methods to assess antimicrobial efficacy.

    Purpose of the Study:

    • To develop and validate a rapid radiometric assay for predicting bacteriostatic endpoints.
    • To compare the performance of the radiometric method against the standard tube dilution method.
    • To assess the utility of the radiometric method for clinical serum samples.

    Main Methods:

    • A mixture of radiolabeled glucose, arginine, and glycine was used to monitor metabolic activity.
    • The radiometric assay involved serial dilutions of clinical samples in Mueller-Hinton broth.

    Related Experiment Videos

  • Antibiotics were inactivated in control samples; 14CO2 production measured after incubation.

    • Main Results:

    • The radiometric method demonstrated high accuracy in predicting bacteriostatic endpoints.
    • A threshold of >= 60% inhibition at a 1:8 dilution correctly predicted tube dilution results in 16 out of 18 samples.
    • The method showed good correlation with established antimicrobial susceptibility testing.

    Conclusions:

    • The 4-hour radiometric method is a viable, rapid alternative for determining antibiotic bacteriostatic endpoints.
    • This assay can be applied to both gram-positive and gram-negative organisms.
    • The developed method offers potential for faster clinical decision-making in infectious disease management.