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Intraocular penetration of rosoxacin in rabbits
Abstract:
Rosoxacin, a new synthetic antimicrobial agent, has a wide spectrum of activity that may prove beneficial in the treatment of ocular infections. To determine the penetration of rosoxacin into ocular tissues and serum of the rabbit after topical, subconjunctival, and intravenous (IV) administration, rosoxacin levels were measured using a microbiological assay after enzymatic digestion of the ocular tissues. Quantities of rosoxacin that should prove to be of therapeutic value were detectable in the anterior segment of the eye after topical or subconjunctival administration. Subconjunctival administration also resulted in high levels in the retina and choroid area and in the optic nerve. Low levels were detected in the retina and choroid 15 minutes following IV injection; however, no detectable levels were found in the ocular tissues after this period.
Insights
Rosoxacin effectively penetrates ocular tissues for treating eye infections. Subconjunctival administration yields therapeutic levels in the anterior segment, retina, and optic nerve.
Area of Science:
- Ophthalmology
- Pharmacology
- Microbiology
Background:
- Ocular infections pose a significant challenge to vision.
- New antimicrobial agents with broad-spectrum activity are needed.
- Rosoxacin is a novel synthetic antimicrobial with potential for treating eye infections.
Purpose of the Study:
- To evaluate the penetration of rosoxacin into ocular tissues and serum.
- To compare rosoxacin levels after topical, subconjunctival, and intravenous administration in rabbits.
- To determine the therapeutic potential of rosoxacin in ocular infections.
Main Methods:
- Rabbit models were used to assess rosoxacin distribution.
- Ocular tissues and serum were analyzed for rosoxacin concentrations.
- A microbiological assay with enzymatic digestion was employed for quantification.
Main Results:
- Therapeutic levels of rosoxacin were detected in the anterior segment after topical and subconjunctival administration.
- Subconjunctival injection led to high rosoxacin concentrations in the retina, choroid, and optic nerve.
- Intravenous administration resulted in low, transient levels in the retina and choroid, with no detectable levels in other ocular tissues post-15 minutes.
Conclusions:
- Rosoxacin demonstrates promising ocular penetration, particularly via subconjunctival administration.
- Subconjunctival rosoxacin may be a viable treatment option for various ocular infections.
- Further studies are warranted to establish optimal dosing and efficacy in clinical settings.