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Monoclonal antibody to human thyrotropin
The Journal of Clinical Endocrinology and Metabolism
|July 1, 1982
Summary
Researchers developed a specific monoclonal antibody for human TSH (hTSH) using somatic cell fusion. This antibody shows high specificity, making it valuable for detecting hTSH-secreting cells and purifying hTSH.
Area of Science:
- Immunology
- Endocrinology
- Biochemistry
Background:
- Monoclonal antibodies are crucial tools in biological research and diagnostics.
- Human TSH (hTSH) is a key hormone in thyroid function, and specific antibodies are needed for its study.
Purpose of the Study:
- To produce and characterize a monoclonal antibody against human TSH (hTSH).
- To evaluate the specificity and affinity of the generated monoclonal antibody.
Main Methods:
- Somatic cell fusion technique was employed to generate hybridomas.
- The affinity of the monoclonal antibody (anti-hTSH 1/1) for hTSH was determined using equilibrium dissociation constants (Kd).
- Cross-reactivity studies were performed with related hormones like hCG and human LH.
Main Results:
- A monoclonal antibody, anti-hTSH 1/1, was successfully produced.
- The antibody exhibited high specificity for hTSH, with a Kd of 4.04 x 10(-9) M/liter.
- Minimal cross-reactivity was observed with the beta-subunit of hTSH, and no cross-reactivity with hCG or human LH.
Conclusions:
- The developed monoclonal antibody (anti-hTSH 1/1) is highly specific for hTSH.
- Its specificity makes it a promising tool for immunofluorescence detection of hTSH-secreting cells.
- The antibody can also be utilized for immunoabsorption and purification of hTSH.