Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Specificity of sperm-zona interaction

C E Swenson, B S Dunbar

    The Journal of Experimental Zoology
    |January 10, 1982
    PubMed
    Summary
    This summary is machine-generated.

    Spermatozoa can bind to the zona pellucida non-specifically or specifically. Using different species in sperm-zona binding assays helps differentiate these interactions, crucial for understanding fertilization.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Omega 3 and omega 6 fatty acids in human and animal health: an African perspective.

    Molecular and cellular endocrinology·2014
    Same author

    Pharmacokinetics of CPX-351; a nano-scale liposomal fixed molar ratio formulation of cytarabine:daunorubicin, in patients with advanced leukemia.

    Leukemia research·2012
    Same author

    Molecular approaches for the evaluation of immune responses to zona pellucida (ZP) and development of second-generation ZP vaccines.

    Reproduction (Cambridge, England). Supplement·2002
    Same author

    Molecular analysis of a carbohydrate antigen involved in the structure and function of zona pellucida glycoproteins.

    Biology of reproduction·2001
    Same author

    Synergistic roles of bone morphogenetic protein 15 and growth differentiation factor 9 in ovarian function.

    Molecular endocrinology (Baltimore, Md.)·2001
    Same author

    Human sperm-oocyte recognition and infertility.

    Seminars in reproductive medicine·2001

    Area of Science:

    • Reproductive Biology
    • Cell Biology
    • Gamete Interactions

    Background:

    • Sperm-zona pellucida interaction is a critical step in fertilization.
    • Understanding the specificity of this interaction is key to reproductive success.

    Purpose of the Study:

    • To investigate the specificity of early sperm-zona interactions in vitro across different species.
    • To differentiate between specific and non-specific sperm binding mechanisms.

    Main Methods:

    • In vitro fertilization assays using gametes from mouse, rabbit, pig, and human.
    • Scanning electron microscopy (SEM) to visualize sperm-zona binding.
    • Sperm-zona binding assays in homologous and heterologous systems.
    • Assessment of calcium dependency for sperm-zona interactions.

    Related Experiment Videos

    Main Results:

    • Mouse spermatozoa showed specific binding to homologous zonae, dependent on capacitation and calcium.
    • Mouse, rabbit, and pig spermatozoa exhibited non-specific binding to heterologous zonae, independent of calcium.
    • Rabbit spermatozoa attached readily to homologous and heterologous zonae.
    • Human spermatozoa did not adhere to heterologous zonae.

    Conclusions:

    • Spermatozoa exhibit both specific and non-specific binding to the zona pellucucida.
    • Heterologous sperm-zona binding assays are valuable tools for distinguishing specific from non-specific interactions.
    • These findings have implications for understanding fertilization mechanisms and potential infertility factors.