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[The larva precipitation test with Ascaris suum and Toxocara canis larvae]
Abstract:
Investigations were made by means of a larvae precipitation test (LPT) on different laboratory animals (pigs and white mice, and pigs, rabbits, guinea pigs and white mice respectively) experimentally infected with Ascaris suum and Toxocara canis respectively for the serological evidence of specific A. suum and T. canis antibodies. Living A. suum larvae isolated from the lungs of experimentally infected white mice forming oral, excretion porus and cuticula precipitates proved to be a suitable antigen for the intravital evidence of prepatent A. suum infections in pigs and mice. By using living T. canis larvae from lungs, liver and brain of infected mice as antigen prepatent T. canis infections in pigs, rabbits, guinea pigs and mice were serologically proved because of precipitates on oral and excretion porus. It was found for the first time that frozen preserved larvae of A. suum and T. canis are suitable for the evidence of specific antibodies and--because of the faster and more exact determination of the results as well as the possible preserving of the antigen--superior to the test on living larvae.