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Bacteriocin production by Streptococcus milleri

D B Drucker, C M McKillop

    Canadian Journal of Microbiology
    |March 1, 1982
    PubMed
    Summary
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    Streptococcus mutans strains exhibit bacteriocinlike activity, often enhanced by glucose. This activity, primarily hydrogen peroxide, inhibits other streptococci, notably Streptococcus milleri.

    Area of Science:

    • Microbiology
    • Bacteriology
    • Antimicrobial Agents

    Background:

    • Investigating bacteriocinlike activity in streptococcal strains is crucial for understanding inter-bacterial competition.
    • Streptococcus mutans and Streptococcus milleri are significant oral bacteria with potential for antagonistic interactions.

    Purpose of the Study:

    • To screen 58 streptococcal strains for bacteriocinlike antagonist production against 50 indicator strains.
    • To identify the nature of the antagonist activity and factors influencing its production.

    Main Methods:

    • Deferred antagonism technique was employed to assess inhibitory activity.
    • Brain heart infusion agar supplemented with glucose and calcium carbonate was used to optimize antagonist production.
    • Exclusion of bacteriophage activity and toxic byproducts (lactate, acetate, ethanol) was performed.

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    Main Results:

    • Bacteriocinlike activity was detected in tested streptococcal strains.
    • Glucose supplementation generally increased antagonist production, while calcium carbonate reduced it.
    • Hydrogen peroxide was identified as the primary agent responsible for antagonist activity in Streptococcus mutans and some S. milleri strains.
    • Most S. milleri isolates were sensitive to the bacteriocinlike activity produced by S. mutans NCTC 10832.

    Conclusions:

    • Streptococcus mutans produces bacteriocinlike substances, primarily hydrogen peroxide, with inhibitory effects on other streptococci.
    • Environmental factors like glucose and calcium carbonate influence the production of these antimicrobial agents.
    • The sensitivity of S. milleri to S. mutans activity highlights potential ecological interactions within microbial communities.