Studies on leucocyte-drug interaction with benoxaprofen

Studies of the preferential action of benoxaprofen on the movement of mononuclear leucocytes have been extended to include human cells, and the positive correlation between human and animal findings is discussed. Benoxaprofen also affected the increased response of the hexose monophosphate (HMP) shunt of rat mononuclear cells stimulated by addition of latex particles but did not inhibit the same metabolic pathway in polymorphonuclear cells. The small effects on phagocytosis by unelicited mononuclear cells did not correlate with the partial inhibition of their HMP shunt by benoxaprofen. Attempts to explain these findings in terms of drug binding were undertaken. High affinity and low affinity binding of benoxaprofen to mononuclear and polymorphonuclear cells were found to occur. Overall, the amount of benoxaprofen bound to mononuclear cells was greater than that bound to polymorphonuclear cells.