Effects of nutrients present in bold's basal medium on the green alga Stigeoclonium pascheri

The effects of varying concentrations of nutrients present in Bold's basal medium on the extent of colony formation from vegetative fragments, sporulation and spore germination of the green alga Stigeoclonium pascheri were studied. A decrease of colony formation was observed in media deficient in MgSO4, NaNO3, phosphates, and containing a 10-fold increase of H3BO3. Sporulation decreased in the same media. However, sporulation was greater in an increasing order in media containing 2- to 10-fold increase in MgSO4. There was a decrease in spore germination in media deficient in phosphates, MgSO4, containing 5- or 10-fold MgSO4, or containing 2- to 10-fold of CaCl2, H3BO3 or microelements. Spore germination increased in media containing 2-fold MgSO4, deficient in H3BO3 or microelements or containing none of the three micronutrient solutions.