Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Probe for polyanionic regions on the cell surface

L Noronha-Blob, J Pitha

    In Vitro
    |February 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    PolyU-proflavine probe estimates cell surface polyanionic regions. Cell density, not transformation or metabolic disorders, significantly impacts probe binding, with aged cells showing higher binding.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Cardiovascular Effects of Early and Postponed Statin Treatment After Ovariectomy in Prediabetic Rat.

    Physiological research·2026
    Same author

    Soluble endoglin reflects endothelial dysfunction in myocardial infarction patients: a retrospective observational study.

    International journal of medical sciences·2025
    Same author

    Hypertension after the Menopause: What Can We Learn from Experimental Studies?

    Physiological research·2023
    Same author

    Acute responses of hepatic fat content to consuming fat, glucose and fructose alone and in combination in non-obese non-diabetic individuals with non-alcoholic fatty liver disease.

    Journal of physiology and pharmacology : an official journal of the Polish Physiological Society·2021
    Same author

    The role of connexin 37 polymorphism in spontaneous abortion.

    Physiological research·2021
    Same author

    Intraventricular placement of a spring expander does not attenuate cardiac atrophy of the healthy heart induced by unloading via heterotopic heart transplantation.

    Physiological research·2019
    Same journal

    New perspectives in hormone-dependent (responsive) and autonomous mammary tumor growth: role of autostimulatory growth factors.

    In vitro·1984
    Same journal

    Comparison of prometaphase chromosome techniques with emphasis on the role of colcemid.

    In vitro·1984
    Same journal

    Effect of serum on organogenesis of the rat testis in vitro.

    In vitro·1984
    Same journal

    Growth characteristics, morphology, and phospholipid composition of human type II pulmonary alveolar cells grown in a collagen-free microenvironment.

    In vitro·1984
    Same journal

    Changes in cytoplasmic and lysosomal enzyme activities in cultured rat heart cells: the relationship to cell differentiation and cell population in culture.

    In vitro·1984
    Same journal

    Drosophila cells and ecdysterone: a model system for gene regulation.

    In vitro·1984
    See all related articles

    Area of Science:

    • Cell Biology
    • Biochemistry
    • Surface Chemistry

    Background:

    • Cell surface analysis requires specific probes for exposed regions.
    • Polyanionic sites are crucial for cell-cell interactions and signaling.
    • Understanding cell surface properties is vital in disease and aging research.

    Purpose of the Study:

    • To develop and validate polyuridylate-proflavine as a probe for cell surface polyanionic regions.
    • To investigate how cell transformation and density affect polyanionic site exposure.
    • To compare probe binding across normal, metabolically disordered, and virus-transformed human fibroblasts.

    Main Methods:

    • Utilizing polyuridylate and proflavine as a binding probe for cell surface analysis.
    • Quantifying probe binding to confluent and sub-confluent cell monolayers.

    Related Experiment Videos

  • Comparing probe binding in normal, metabolically disordered, and simian virus 40-transformed human fibroblasts.
  • Assessing probe binding in aged in vitro fibroblast populations.

    • Main Results:

    • The polyU-proflavine complex preferentially binds to hydrophilic, polyanionic cell surface regions.
    • Virus-transformed fibroblasts bind significantly less probe than normal cells at confluence.
    • Probe binding increases dramatically with decreased cell density for both normal and transformed cells.
    • Fibroblasts from normal donors, metabolic disorder patients, and virus-transformed cells show similar binding at equivalent densities.
    • Aged fibroblasts, characterized by larger cell size and lower density, exhibit disproportionately high probe binding.

    Conclusions:

    • The polyU-proflavine complex is a reliable probe for estimating exposed polyanionic regions on cell surfaces.
    • Cell density is a critical factor influencing polyanionic site accessibility, overriding transformation status or metabolic disorders.
    • Aging in vitro leads to alterations in cell surface polyanionic composition, increasing probe accessibility.