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Characterization of a cell cycle mutant derived from hamster fibroblast: reversion analysis
The Journal of Cell Biology
|March 1, 1982
Summary
A temperature-sensitive (ts) mutant cell line, K12, showed defects in DNA synthesis and protein production. A revertant clone, R12, regained normal growth, indicating the K12 mutation is regulatory.
Area of Science:
- Cell Biology
- Genetics
- Molecular Biology
Background:
- K12 is a Chinese hamster fibroblast cell line with temperature-sensitive (ts) mutations.
- At nonpermissive temperatures, K12 cells display inhibited DNA synthesis, suppressed thymidine kinase, and enhanced synthesis of specific proteins (94, 78, 58 kdaltons).
Purpose of the Study:
- To characterize a spontaneous revertant clone (R12) derived from the K12 cell line.
- To investigate the nature of the ts mutation in K12 cells by analyzing the revertant phenotype.
Main Methods:
- Isolation and characterization of a spontaneous revertant clone (R12) from K12 cells.
- Selection of R12 based on its ability to proliferate at the nonpermissive temperature.
Main Results:
- The R12 clone exhibited simultaneous reversion of all K12 mutant traits to the wild-type state.
- Growth at the nonpermissive temperature was restored in the R12 cell line.
Conclusions:
- The K12 ts mutation appears to be regulatory, affecting multiple cellular phenotypes.
- The simultaneous reversion of all traits in R12 supports a single regulatory mutation model.