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Relationship between adherence, chemotaxis and the accumulation of rat polymorphonuclear leukocytes at an
Abstract:
The effects of a nonsteroidal anti-inflammatory agent, indomethacin, a steroidal anti-inflammatory drug, dexamethasone and an antirheumatic agent, levamisole, on rat polymorphonuclear leukocyte (PMN) adherence and migration were examined. Adherence was evaluated using nylon fiber columns and migration was studied in vitro using Boyden chambers In addition, the effects of these drugs on PMN accumulation in he carrageenan-injected pleural cavity was evaluated. PMNs removed from animals treated with indomethacin, 1 mg/kg p.o., had a significantly reduced adherence, and fewer cells accumulated at an inflammatory site. Cellular migration measured in vitro was unaffected. PMNs removed from animals treated with dexamethasone, 0.1 and 1.0 mg/kg p.o., had a significantly reduced adherence and fewer cells accumulated at an inflammatory site. Cell migration was not consistently affected. After treatment with levamisole, 5 and 25 mg/kg p.o., adherence and chemotaxis were reduced, whereas PMN accumulation was unaffected. These results demonstrate that no simple relationship exists between PMN adherence, chemotaxis and their accumulation at an inflammatory site. Neither a reduction in adherence nor migration, as measured in vitro with a Boyden chamber, was predictive of a change in inflammatory cell migration.
Insights
Nonsteroidal anti-inflammatory drugs (NSAIDs) and steroidal anti-inflammatory drugs reduced polymorphonuclear leukocyte (PMN) adherence and inflammatory cell accumulation. Antirheumatic agents affected PMN adherence and chemotaxis but not accumulation, indicating complex relationships.
Area of Science:
- Pharmacology
- Immunology
- Inflammation Research
Background:
- Polymorphonuclear leukocytes (PMNs) play a crucial role in inflammatory responses.
- Understanding how anti-inflammatory and antirheumatic agents affect PMN function is vital for treating inflammatory diseases.
Purpose of the Study:
- To investigate the effects of indomethacin, dexamethasone, and levamisole on rat PMN adherence, migration, and in vivo accumulation at an inflammatory site.
Main Methods:
- PMN adherence was assessed using nylon fiber columns.
- In vitro PMN migration was studied using Boyden chambers.
- In vivo PMN accumulation was evaluated in a carrageenan-induced pleural inflammation model.
Main Results:
- Indomethacin and dexamethasone significantly reduced PMN adherence and inflammatory cell accumulation.
- Levamisole decreased PMN adherence and chemotaxis but did not affect inflammatory cell accumulation.
- In vitro migration assays did not consistently predict in vivo PMN accumulation.
Conclusions:
- No straightforward correlation exists between PMN adherence, chemotaxis, and inflammatory cell accumulation.
- Reduced PMN adherence or in vitro migration does not necessarily predict altered inflammatory cell migration in vivo.