Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Human spectrin. II. An electro-optic study

A Mikkelsen, A Elgsaeter

    Biochimica Et Biophysica Acta
    |September 26, 1978
    PubMed
    Summary
    This summary is machine-generated.

    Human spectrin heterodimers show significant electrically induced birefringence, revealing a contour length exceeding 50 nm. This electro-optic study provides key insights into spectrin

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Esophageal atresia and parental psychological distress in the neonatal period.

    Journal of pediatric surgery·2026
    Same author

    Bismuth-oxide nanoparticles: study in a beam and as deposited.

    Physical chemistry chemical physics : PCCP·2024
    Same author

    Structural and chemical properties of anion exchanged CsPb(Br<sub>(1-</sub>Cl<sub></sub>)<sub>3</sub>heterostructured perovskite nanowires imaged by nanofocused x-rays.

    Nanotechnology·2024
    Same author

    Prenatal Diagnosis of Esophageal Atresia - Performance and Consequences.

    Journal of pediatric surgery·2023
    Same author

    Electric-field-induced deformation, yielding, and crumpling of jammed particle shells formed on non-spherical Pickering droplets.

    Soft matter·2021
    Same author

    Electrorotation of particle-coated droplets: from fundamentals to applications.

    Soft matter·2021
    Same journal

    Cumulative Contents.

    Biochimica et biophysica acta·2020
    Same journal

    Molecular Basis of Disease Cumulative Contents.

    Biochimica et biophysica acta·2020
    Same journal

    General Subjects Cumulative Contents.

    Biochimica et biophysica acta·2020
    Same journal

    Erratum to 'on the role of exchangeable hydrogen bonds for the kinetics of P680<sup>+·</sup> Q<sub>A</sub> <sup>-·</sup> formation and P680<sup>+·</sup> Pheo<sup>-·</sup> recombination in photosystem II' [Biochim. Biophys. Acta 1276 (1996) 35-44].

    Biochimica et biophysica acta·2019
    Same journal

    Oligomeric state of the light-harvesting complexes B800-850 and B875 from purple bacterium Rubrivivax gelatinosus in detergent solution.

    Biochimica et biophysica acta·2019
    Same journal

    Regulation of pigment content and enzyme activity in the cyanobacterium Nostoc sp. Mac grown in continuous light, a light-dark photoperiod, or darkness.

    Biochimica et biophysica acta·2019
    See all related articles

    Area of Science:

    • Biophysics
    • Molecular Biology
    • Materials Science

    Background:

    • Spectrin is a crucial protein in the cytoskeleton of red blood cells and other cell types.
    • Understanding the physical properties of spectrin is essential for comprehending cell mechanics and structure.
    • Electro-optic techniques offer sensitive methods for probing macromolecular conformation and dynamics.

    Purpose of the Study:

    • To investigate the electrically induced birefringence of human spectrin heterodimers.
    • To determine key electro-optical parameters, including the Kerr constant and relaxation time.
    • To estimate the molecular dimensions, specifically the contour length, of human spectrin heterodimers.

    Main Methods:

    • Solutions of human spectrin heterodimers were subjected to an electric field.

    Related Experiment Videos

  • Electrically induced birefringence was measured to analyze molecular responses.
  • Birefringence decay was monitored to calculate relaxation times.

    • Main Results:

    • A specific Kerr constant (Bsp) of +(5 +/- 2) x 10(-11) m4/(V2 . kg) was determined for human spectrin heterodimers.
    • A birefringence relaxation time (tau decay) of (2.0 +/- 0.3) microseconds was observed.
    • The electro-optic data suggest a contour length for human spectrin heterodimers greater than 40-50 nm.

    Conclusions:

    • Human spectrin heterodimers exhibit significant electro-optic properties.
    • The determined Kerr constant and relaxation time provide insights into spectrin's electrical polarizability and rotational dynamics.
    • The estimated contour length aligns with spectrin's known role as a large, elongated structural protein.