Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Evidence that patterning mechanisms in developing and regenerating limbs are the same

K Muneoka, S V Bryant

    Nature
    |July 22, 1982
    PubMed
    Summary

    Amphibian limb regeneration and development share similar patterning mechanisms. Experiments with axolotl grafts show that developing and regenerating limbs respond similarly, indicating conserved developmental processes.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Identification of Heparan-Sulfate Rich Cells in the Loose Connective Tissues of the Axolotl (Ambystoma mexicanum) with the Potential to Mediate Growth Factor Signaling during Regeneration.

    Regenerative engineering and translational medicine·2021
    Same author

    Improvement of mutism in a catatonic schizophrenia case by add-on treatment with amantadine.

    Pharmacopsychiatry·2010
    Same author

    Neurotrophic regulation of epidermal dedifferentiation during wound healing and limb regeneration in the axolotl (Ambystoma mexicanum).

    Developmental biology·2008
    Same author

    Inhibition of polarizing activity in the anterior limb bud is regulated by extracellular factors.

    Developmental biology·2002
    Same author

    Nicotine exposure during pregnancy is a factor which influences serotonin transporter density in the rat brain.

    European journal of pharmacology·2001
    Same author

    Expression of Hoxb13 and Hoxc10 in developing and regenerating Axolotl limbs and tails.

    Developmental biology·2001

    Area of Science:

    • Developmental Biology
    • Regenerative Medicine
    • Amphibian Biology

    Background:

    • Amphibians, like the axolotl (Ambystoma mexicanum), possess remarkable limb regeneration capabilities.
    • The processes of limb development and regeneration share similarities in pattern formation.
    • Debate exists on whether these processes utilize identical or distinct underlying mechanisms.

    Purpose of the Study:

    • To investigate the mechanistic similarities between limb development and regeneration in amphibians.
    • To determine if conserved patterning mechanisms govern both processes.
    • To test the hypothesis that limb development and regeneration employ similar cellular and molecular pathways.

    Main Methods:

    • Utilizing grafting experiments in the axolotl model system.
    • Misaligning tissues within developing and regenerating limbs to induce supernumerary structures.
    • Grafting tissues between developing and regenerating limbs to observe cross-system responses.

    Main Results:

    • Both developing and regenerating axolotl limbs formed supernumerary outgrowths when subjected to tissue misalignment.
    • Grafts of regenerating limb tissue into developing limbs, and vice versa, resulted in the formation of supernumerary structures.
    • The responses observed were organized, suggesting functional integration rather than disorganization.

    Conclusions:

    • The patterning mechanisms underlying limb development and regeneration in the axolotl are fundamentally the same.
    • These findings support the conservation of developmental pathways between initial limb formation and subsequent regeneration.
    • This suggests that regenerative processes may leverage existing developmental toolkits.

    Related Experiment Videos