[Growth of myeloid plaques on layers of cultured stromal fibroblasts]

The cells of syngeneic bone marrow were added in the primary monolayer cultures of bone marrow, thymus and peritoneal fluid of the mouse. Discrete plaques with several dozens to several thousands of myeloid cells developed on the surface of fibroblast colonies within 2--7 days. It was shown that both the size and rate of formation of myeloid plaques were markedly higher in the culture of thymus fibroblasts than in those of bone marrow and peritoneal fluid fibroblasts. Hence, the stromal fibroblasts of different origin differ from each other by their ability to interact in vitro with hemopoietic precursor cells forming myeloid plaques.