[Effect of extracts of different origin on primary cultures of cells from inbred mice]

The effect of allogeneic and syngeneic extracts from inbred male and female mouse spleens on the primary culture of fibroblasts obtained from subcutaneous connective tissue of CBA and C57BL/6j female mouse fetus was studied. Cytotoxic and growth-inhibiting effect on the cultures increased successively in using extracts from the tissues of syngeneic male mice, allogeneic female and male mice. Consequently, an increase in the degree of antigenic differences of the target=cells and extracts led to enhancement of the allogeneic inhibition phenomenon. For the first time it was shown that in the syngeneic system the extracts from male mouse tissues (containing weak H-Y antigen) exerted a cytotoxic action on the inbred female mouse cells, i.e. caused reaction similar to allogeneic inhibition.