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Cytotoxic material released from Staphylococcus epidermidis. I. Effects on [3H]thymidine incorporation of human

M Solymossy, Z Nagy, F Antoni

    Acta Microbiologica Academiae Scientiarum Hungaricae
    |January 1, 1982
    PubMed

    Incorporation of [3H]thymidine ([3H]TdR) into tonsil lymphocytes was inhibited by native Staphylococcus epidermidis while Staphylococcus aureus Cowan I caused stimulation. The inhibitory effect of S. epidermidis was abolished by formalin treatment but not by heat killing. A toxic agent was released from S. epidermidis on gentle water extraction without lysing the bacteria. The extract contained protein and other UV-absorbing material, but did not exhibit haemolytic, lysozyme, catalase or protease activity. The heat-resistant, formalin-sensitive inhibitor present in the aqueous extract of S. epidermidis inhibited [3H]TdR incorporation of lymphocytes in a dose-dependent manner and decreased the viability of lymphocytes.

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