Regional blood flows measured in Mongolian gerbil by a modified microsphere method

Area of Science:

• Physiology
• Cardiovascular Research

Background:

• Gerbils are extensively used in stroke research.
• Accurate hemodynamic measurements are vital for understanding stroke mechanisms.
• Existing methods may not be optimal for gerbil hemodynamic studies.

Purpose of the Study:

• To adapt and validate a microsphere method for determining regional blood flow in gerbils.
• To assess the applicability of this method for measuring organ and cerebral blood flow.
• To facilitate hemodynamic research in gerbils for stroke studies.

Main Methods:

• A modified microsphere method using carbonized microspheres (15 micrometers) labeled with radioactive isotopes.
• Injection into the left ventricle and reference sample withdrawal from tail artery cannula in anesthetized gerbils.
• Validation of microsphere mixing, pulmonary/systemic extraction, and hemodynamic stability.

Main Results:

• The microsphere method demonstrated nearly 100% extraction by capillary beds.
• No significant hemodynamic alterations were observed during the procedure.
• Regional blood flow values obtained were comparable to those previously reported in rats.
• The reference sample method was deemed applicable to gerbils.

Conclusions:

• The validated microsphere method is suitable for measuring organ and regional cerebral blood flow in gerbils.
• This technique enhances the utility of gerbils in hemodynamic research, particularly for stroke studies.
• The study provides a reliable method for future investigations into gerbil physiology and disease models.