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Effect of anti-HCG sera on purified syncytiotrophoblast from full-term human placenta
Heterologous anti-HCG and anti-beta-HCG sera failed to effect complement-dependent lysis of purified 51chromium-labeled syncytiotrophoblast in vitro. The progesterone synthetic activity of syncytiotrophoblast was also preserved in the presence of the anti-HCG sera. Binding of the anti-HCG antibodies to the syncytiotrophoblast was demonstrated in an immunofluorescence test. The potential of the anti-HCG sera to participate in complement dependent lysis of other HCG-bearing cell types was revealed using BeWo cells as the targets; 53%-68% of the latter were killed by exposure to anti-HCG sera and complement. The possible mechanism(s) whereby syncytiotrophoblast resists damage by the anti-HCG sera are considered.
Heterologous anti-HCG and anti-beta-HCG sera failed to effect complement-dependent lysis of purified 51chromium-labeled syncytiotrophoblast in vitro. The progesterone synthetic activity of syncytiotrophoblast was also preserved in the presence of the anti-HCG sera. Binding of the anti-HCG antibodies to the syncytiotrophoblast was demonstrated in an immunofluorescence test. The potential of the anti-HCG sera to participate in complement dependent lysis of other HCG-bearing cell types was revealed using BeWo cells as the targets; 53%-68% of the latter were killed by exposure to anti-HCG sera and complement. The possible mechanism(s) whereby syncytiotrophoblast resists damage by the anti-HCG sera are considered.