[Interaction between the fluorescent probe 1-anilino-naphthalene-8-sulfonate and chloroplasts]

Interaction between fluorescent probe 1-anilino-naphthalene-8-sulphonate (ANS) and chloroplasts was studied. Parameters of ANS binding with membranes and weakly-bound proteins of chloroplasts were determined. It has been shown that at all pH values the increase of quantum yield of ANS fluorescence with the addition of chloroplasts is mainly determined by the interaction between the probe and weakly-bound chloroplast proteins.