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Related Experiment Videos

Opioid peptides decrease calcium-dependent action potential duration of mouse dorsal root ganglion neurons in cell

M A Werz, R L Macdonald

    Brain Research
    |May 6, 1982
    PubMed
    Summary

    Leucine-enkephalin, an opioid peptide, reduces calcium-dependent action potentials in dorsal root ganglion (DRG) neurons. This action, mediated by opiate receptors, suggests similar receptor function on neuron somata and primary afferent terminals.

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    Area of Science:

    • Neuroscience
    • Pharmacology
    • Cell Biology

    Background:

    • Opioid peptides modulate neuronal excitability.
    • Dorsal root ganglion (DRG) neurons are crucial for pain signaling.
    • Somatic and terminal opiate receptor functions require clarification.

    Purpose of the Study:

    • To investigate the effects of opioid peptides on DRG neuron somatic action potentials.
    • To determine the involvement of opiate receptors in these effects.
    • To compare the function of somatic opiate receptors with those on primary afferent terminals.

    Main Methods:

    • Primary dissociated cell culture of DRG neurons.
    • Electrophysiological recordings of somatic calcium-dependent action potentials.
    • Dose-response studies with leucine-enkephalin.

    Related Experiment Videos

  • Antagonism studies using naloxone.

    • Main Results:

    • Leucine-enkephalin significantly decreased the duration and amplitude of DRG somatic action potentials.
    • The effect was dose-dependent, occurring between 20 nM and 5 microM.
    • Naloxone antagonized the action of leucine-enkephalin, confirming opiate receptor mediation.
    • Opioid peptide action reduced calcium influx into DRG neurons.

    Conclusions:

    • DRG neuron somata possess functional opiate receptors.
    • These receptors decrease calcium influx, modulating neuronal excitability.
    • Somatic opiate receptors on DRG neurons are likely functionally similar to those on primary afferent terminals.